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Spectroscopic Method: HPLC
Substituent: Nitro
HPLC Chromatogram of (3E)-3-(4-Nitrobenzylidene)-1,3-dihydro-2H-indol-2-one:
The sharp peak at the retention time of 6.074 confirms the presence of a single isomer in this compound while the small peak at 5.832 retention time indicates trace of impurities. The percentage area peak of this compound confirms its percentage yield to be 67.58% against its calculated value of 70%.
Substituent: Nitro
HPLC Chromatogram of (3E)-3-(4-Nitrobenzylidene)-1,3-dihydro-2H-indol-2-one:
The sharp peak at the retention time of 6.074 confirms the presence of a single isomer in this compound while the small peak at 5.832 retention time indicates trace of impurities. The percentage area peak of this compound confirms its percentage yield to be 67.58% against its calculated value of 70%.